A novel approach to sperm selection: Nanoparticle-based purification improves quality of Angora cryopreserved buck’s semen

The Aim: aim of the study was to investigate applicability an up-to-date method, sperm selection with nanoparticles at different temperatures (37ºC, 21ºC, and 4ºC), on Angora buck semen. Material Methods: Semen samples were collected from 3 bucks 7 repetitions each sample diluted divided into 4 groups. Nanopurification procedure applied by using a nanoparticle dose 10 μg/ml three groups 37ºC, 4ºC while in control group cryopreserved without nanopurificiation treatment. Post-thaw motility morphology parameters analyzed computer-aided analysis (CASA) fluorescein microscopy.Results: After nanopurification 37ºC total motility, linearity (LIN) (P<0.05), curvilinear velocity (VCL), straight-line (VSL), acrosome integrity (P<0.001) higher than semen nanopurified 21ºC superior terms LIN, straightness (STR) VCL, VSL, average path (VAP), wobble (P<0.001). There no significant difference between group.Conclusion: In conclusion, useful that affect fertility method can be performed as well adapt field conditions;